Acetylcholine Receptor Autoantibody ELISA kit ELISA

  • Photography of ACHRAB ELISA
  • Photography of ACHRAB ELISA
The ACHRAb kit is an accurate enzymatic assay used to measure acetylcholine receptor autoantibodies (AChRAb) in human serum in relation to myasthenia gravis.
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The ACHRAb kit is an accurate enzymatic assay used to measure acetylcholine receptor autoantibodies (AChRAb) in human serum in relation to myasthenia gravis.
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Overview

Contributes to the diagnosis of neuromuscular junction failure in myasthenia gravis (Erb-Goldflam disease)

Monitor myasthenia gravis

Benefits

  • ACCURATE AND RELIABLE ENZYMATIC ASSAY

Assay principle: competition

The assay is based on competition between AChRAb from the human serum and MAb1 (coated on the microplate), and/or MAb2 and/or MAb3 (labeled with biotin) in binding to the Acetylcholine Receptor (AChR). The bound MAb2 and MAb3-biotin are detected by the addition of Streptavidin Peroxidase (SA-POD). The unbound SA-POD in excess is washed off before the addition of the peroxidase substrate TMB.

Assay procedure: 16h at 2-8°C + 1 h at RT / shaking + 1 h at RT / shaking + 30 min at RT / shaking + 30 min at RT in the dark.

Features

Working range: 0.5 – 20 nmol/L

Sample: serum – 100 µL

Analytical sensitivity: 0.25 nmol/L

Normal values

Cut-offAChRAb nmol/L
Negative<0.45
Positive>= 0.45