Alpha-SMA kit
Alpha-SMA quantification in cell lysates
Amyloid beta1-40 Kit HTRF®
The Amyloid ß1-40 kit is designed for the simple and rapid quantification of the peptide in cell-based formats.
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Ready-to-use
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Highly accurate
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Faster and more convenient than ELISA
Overview
Amyloid ß1-40 and ß1-42 peptides are involved in the appearance of Alzheimer's disease symptoms. The production of these two amyloid peptides is regulated by the activity of 2 enzymes, ß and γ secretase, which cleave the amyloid precursor protein. This kit is intended to measure amyloid ß1-40 peptides in cell supernatant or in whole cells. This assay is a fast alternative to ELISA, thanks to the ease-of-use of our Add and Read method.
Benefits
- HIGHLY SPECIFIC
- READY-TO-USE KIT
Assay principle
The HTRF Amyloid ß1-40 assay is a sandwich immunoassay using two monoclonal antibodies that recognize different epitopes within the ß1-40 peptide. One antibody is labelled with Eu3+-Cryptate and the other with XL-665. The assay can be used with whole cells or cell supernatant.
Assay Protocol
The Amyloid ß1-40 assay features a streamlined protocol with only two incubation steps:
- Cell stimulation by target compounds
- Amyloid ß1-40 detection using HTRF reagents
The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further speed up the assay procedure. This protocol requires a single 24-hour incubation at 4°C following cell stimulation.
Key features
| Detection limit: | 13.64 pg/mL |
|---|---|
| Dynamic range: | 25 to 1600 pg/mL |
| Sample compatibility: | Cell-based or cell supernatants |
| Specificity: | No cross-reaction with Amyloid ß 1-42 and ß 1-43 |
Product Insert Amyloid Kit / 62B40PEG-62B40PEH
62B40PEG-62B40PEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
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Molecular basis of neuroinflammation and neurodegeneration diseases
The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides
Neurodegeneration and its main related diseases
Discover this infographic design on neurodegenerative diseases - Infographics
On-demand webinar: Linking Neuroinflammation and Neurodegeneration
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Assays for neurosciences research
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Safety Data Sheet Amyloid Kit / 62B40PEG
62B40PEG - Safety Data Sheet
Safety Data Sheet (DEU) Amyloid Kit / 62B40PEG
62B40PEG - Safety Data Sheet
Safety Data Sheet (ELL) Amyloid Kit / 62B40PEG
62B40PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) Amyloid Kit / 62B40PEG
62B40PEG - Safety Data Sheet
Safety Data Sheet (ITA) Amyloid Kit / 62B40PEG
62B40PEG - Safety Data Sheet
Safety Data Sheet (SPA) Amyloid Kit / 62B40PEG
62B40PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) Amyloid Kit / 62B40PEG
62B40PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) Amyloid Kit / 62B40PEG
62B40PEG - Safety Data Sheet
Safety Data Sheet (DEU) Amyloid Kit / 62B40PEH
62B40PEH - Safety Data Sheet
Safety Data Sheet (ELL) Amyloid Kit / 62B40PEH
62B40PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) Amyloid Kit / 62B40PEH
62B40PEH - Safety Data Sheet
Safety Data Sheet (ITA) Amyloid Kit / 62B40PEH
62B40PEH - Safety Data Sheet
Safety Data Sheet (SPA) Amyloid Kit / 62B40PEH
62B40PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) Amyloid Kit / 62B40PEH
62B40PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) Amyloid Kit / 62B40PEH
62B40PEH - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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