Alpha-SMA quantification in cell lysates
Amyloid ß1-40 and ß1-42 peptides are involved in the appearance of Alzheimer's disease symptoms. The production of these two amyloid peptides is regulated by the activity of 2 enzymes, ß and γ secretase, which cleave the amyloid precursor protein. This kit is intended to measure amyloid ß1-40 peptides in cell supernatant or in whole cells. This assay is a fast alternative to ELISA, thanks to the ease-of-use of our Add and Read method.
The Amyloid ß1-40 assay features a streamlined protocol with only two incubation steps:
- Cell stimulation by target compounds
- Amyloid ß1-40 detection using HTRF reagents
The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further speed up the assay procedure. This protocol requires a single 24-hour incubation at 4°C following cell stimulation.
|Detection limit:||13.64 pg/mL|
|Dynamic range:||25 to 1600 pg/mL|
|Sample compatibility:||Cell-based or cell supernatants|
|Specificity:||No cross-reaction with Amyloid ß 1-42 and ß 1-43|
Get the brochure about technology comparison. - Brochures
The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides
Discover this infographic design on neurodegenerative diseases - Infographics
New insight into neuroinflammation research - Videos
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.Let's find your reader
Phospho-STAT3 (Tyr705) cellular kit
All-in-one kit for robust detection of Phospho-STAT3 (Ser423/425)
Discover this blog article to learn more about the neuroinflammation inv...
Development of of an HTRF assay to screen putative b-amyloid inhibitors