DELFIA® technology Basics
A proven and robust immunodetection platform
DELFIA® Assay Principle
DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of an analyte of interest using lanthanide chelate labeled reagents. Wash steps are used to eliminate unbound reagent.
DELFIA assays can be performed in either direct antigen, indirect antibody sandwich, or competitive assay formats. The technology can equally be applied to fixed or adherent cells.
How DELFIA assays work
DELFIA has a similar workflow to that of a traditional ELISA. Let’s take the example of an immuno assay setup with a direct antibody coating.
First the plate is coated with a capture antibody. After an overnight incubation, the plate is washed 3 times. The sample is then added and incubated 1.5 hours before a further 3 washing steps. The detection antibody is incubated for 1.5 hours before 3 more washing steps. At that stage, the analyte is sandwiched between the 2 antibodies. The final addition of an enhancement solution will increase the sensitivity of the assay. The lanthanide chelate is dissociated and a new highly fluorescent chelate is formed within a protective micellar solution. The fluorescence emitted can be measured with a plate reader capable of TRF detection.
How DELFIA can help you in your research
While superficially quite similar to an ELISA, DELFIA comes with additional benefits. DELFIA has a stable, time-resolved fluorescent signal which improves the assay dynamic range. DELFIA also offers a multiplexing capability (ability to measure multiple targets within the same sample).
DELFIA is compatible with complex matrices, such as whole cells, cell membranes, cell lysates, tissue homogenates, serum, plasma, etc. DELFIA covers a wide range of applications from biochemical to cell membrane-based, and cell-based (live or fixed cell) assays.
Long signal stability
Broad sample compatibility
Wide detectable range
DELFIA expands research possibilities
Thanks to its unrivalled robustness, biodistribution analyses can be assessed using samples from tested animals (blood, urine, feces, or tissue homogenates), making DELFIA the most universal non-radioactive technological platform.
From immunoassays to binding and enzymatic studies, to cytotoxicity and cell proliferation assays, DELFIA TRF assays are sure to provide you with unmatched sensitivity!
Greater confidence in your results with powerful microplate readers
PerkinElmer’s portfolio of plate readers provides the latest detection technologies to meet the diverse assay requirements of labs today. DELFIA assays require a plate reader that is capable of TRF (time-resolved fluorescence) detection. VICTOR® Nivo™, EnSight®, or EnVision® all represent trusted solutions to consider in your investment budget.
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