Immunoassay Platforms to Fit Every Need

Be sure to choose the best possible option for success

The right technology choice for a successful assay

When it comes to choosing the right assay for your research, traditional technology almost always gives you the choice between going with ELISA (enzyme-linked immunosorbent assays) or Western Blot.
While these techniques are quite well known and widely used, others can be implemented which will provide you with additional benefits like ease of use, scalability, or the absence of washing, without compromising on sensitivity and specificity.
DELFIA^®, LANCE^®, HTRF^®, or Alpha technologies are among the many options to explore!

	ELISA	DELFIA	HTRF and LANCE Ultra	Alpha
PLATE FORMAT	96-wells	96 to 384-wells	Flexible: Anywhere from 96- to 1536-wells
MINIATURIZATION	Limited	Limited	Yes	Yes
TIME TO RESULTS	4-24 hr	4-6 hr	1-2 hr	1-2 hr
	–	–	Significant time saving reallocated back to science
WASH STEPS	Yes	Yes	No	No
	While timely, in some instances wash steps can be beneficial. Wash steps may become necessary to remove nonbound analytes with higher cross-reactivity profiles and to decrease background		Absence of wash steps increases throughput, results in ease of use, reduces time to results and lowers variability
DYNAMIC RANGE	2 Log	5 Log	4 Log	5 Log
	–	Large dynamic ranges are beneficial to avoid making extremely large dilutions during sample preparation. Large sample dilutions are cumbersome and lead to analytical imprecision/variability
SENSITIVITY	10 pg/mL	10 pg/mL	10 pg/mL	1 pg/mL
	Sufficient sensitivity to assay in serum, fluid, cell supernatant & lysates		Sufficient sensitivity to assay in cell supernatant & lysates	Sufficient sensitivity to assay in cell supernatant & lysates. May lend itself to diluting then testing serum samples, due to its superior sensitivity
AVIDITY	No	No	Only with streptavidin	Yes
	–	–	Avidity may be a sought after characteristic when detecting protein-protein interactions of low affinities
SIGNAL STABILITY	Up to 1 hr	Days – Years	24 hr to days	up to 24 hr
	–	Allows for the plates to be left on the bench and read at an alternate later and more convenient time. Maximizes assay precision when incubation times vary		–
MULTIPLEXING	No	Yes (up to 3 targets)	No	Yes (up to 3 targets)
READER REQUIREMENTS	Chromogenic and Chemiluminescence Compatible	TR-FRET Compatible	TR-FRET Compatible	Alpha Compatible
	–	100+ compatible reader	50+ compatible reader	10+ compatible reader
CHEMISTRY	–	Fluorophore Chelate	Fluorophore Chelate (LANCE Ultra) and Fluorophore Cryptate (HTRF)	Fluorophore Embedded Beads
	–	Extremely robust chemistry, light, temperature insensitive. Ion insensitive (HTRF only)		–
ANALYTE SIZE	No limitation	No limitation	Subject to förster radius (9 nm)	Subject to oxygen singlet diffusion (200 nm)

DELFIA technology

DELFIA assay principle

DELFIA (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology.
DELFIA has a similar assay principle and workflow to that of a traditional ELISA, but with the added benefits of a stable, time-resolved fluorescent signal improving assay dynamic range. DELFIA also offers a multiplexing capability.

Learn more about DELFIA

How DELFIA compares to ELISA

Below is a workflow comparison between a DELFIA TRF assay and a traditional ELISA.

LANCE Ultra technology

LANCE Ultra assay principle

LANCE (Lanthanide Chelate Excite) TR-FRET is a no wash proximity assay that combines the benefits of time resolution (TR) with fluorescence resonance energy transfer (FRET) principles by using energy donor and energy acceptor fluorophore labels.
It’s everything you love about TRF detection with faster time to results and no wash steps.

Learn more about LANCE Ultra

How LANCE Ultra compares to ELISA

Below is a workflow comparison between a LANCE Ultra TR-FRET assay and a traditional ELISA.

HTRF technology

HTRF assay principle

HTRF (Homogeneous Time Resolved Technology) is a FRET-based, no-wash technology.
Rapid, homogeneous, easy to use and to automate, allowing extreme miniaturization and easy assay development – over time, HTRF has become the gold reference for time-resolved FRET.
HTRF technology can be used in competitive and noncompetitive formats, performed as cellular or biochemical assays and thus can been applied to a variety of applications including GPCRs, kinases, epigenetics, and quantification of a range of biomarkers including cytokines.

Learn more about HTRF

How HTRF compares to ELISA

Below is a workflow comparison between an HTRF assay and a traditional ELISA.

Alpha technology

Alpha assay principle

Alpha is a bead-based, no-wash luminescent technology. When Alpha Donor and Acceptor beads are brought together through binding to the target, a cascade of chemical reactions is set in motion, creating an amplified signal. These versatile beads are conjugated with various biomolecules that enable the detection of unique biological events, offering maximum flexibility.

Learn more about Alpha

How Alpha compares to ELISA

Below is a workflow comparison between an Alpha assay and a traditional ELISA.