CXCR4R fluorescent probe
Tag-lite CXCR4 receptor fluorescent probe
CXCR4 is a receptor for the C-X-C chemokine CXCL12/SDF-1. Compelling evidences suggests that CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation. The receptor also plays an essential role in vascularization of the gastrointestinal tract. CXCR4 acts as a coreceptor for human immunodeficiency virus-1/HIV-1.
This HEK293 stable cell line expresses the CXCR4 receptor fused to a SNAP-Tag. Labeled with Terbium, the cells can be used in a Tag-lite receptor binding protocol.
SNAP-tag® is a small fusion tag that covalently interacts with specific substrates. It enables specific covalent labeling of any protein of interest. For more details, see the labeling procedure.
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells, and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Examples of data obtained using chemokine CXCR4-R expressing stable cells (HEK) labelled with terbium in Tag-lite buffer (LABMED), and their matching fluorescent ligand (L0012RED). AMD-3100 was used as reference ligand. Results may vary from one HTRF® compatible reader to another.
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