HTRF Anti-VHH-Tb cryptate HTRF®
HTRF Anti-VHH-Tb cryptate can be used to capture alpaca and llama VHH nanobodies.
- High affinity
Monoclonal anti-VHH antibody has been labeled with Tb crypate. Anti-VHH antibody binds to the native form of both alpaca and llama VHH and recombiant nanobodies. The binding is rapid and stable, making it an ideal choice for use in a variety of assays such as nanobodies selection and titration.
- BATCH-TO-BATCH REPRODUCIBILITY
- LOW TO HIGH KD DETECTION
In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the signal is proportional to the binding of the 2 partners. In the example shown here, anti-VHH-Tb binds to the nanobodies raised against protein A, while partner A* binds to a specific Ab labeled with an HTRF acceptor.
*partner A can also be biotinylated, tagged, or Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with an acceptor for the detection.
The example on the right describes the protocol using a 20 µL final assay volume for the detection of an interaction between a nanobody raised against protein A and a non-tagged partner A*. Dispense the two partners (10 µL): i.e. protein A+nanobody, add anti-VHH-Tb cryptate (total 5 µL), and anti-partner A labeled with acceptor (5 µL) Then incubate, and finally read.
*partner A can also be biotinylated, tagged or Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin), labeled with an acceptor for the detection.
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Plate Reader Requirement
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