HTRF KinEASE-STK S1 kit
A HTRF KinEASE kit to study serine/threonine kinases
HTRF KinEASE STK Discovery kit HTRF®
The HTRF KinEASE assay series is a semi-universal biochemical platform to study kinase activity. The Discovery kit provides a straightforward way to determine which of the STK kits must be used for a given serine/threonine kinase.
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Low enzyme consumption
Overview
The HTRF KinEASE kit series provides a simple biochemical approach to study kinase activity, screen for inhibitors and characterize them. Three STK and one TK kits, offer a semi-universal method for assessing phosphorylation on Serine/Threonine (STK) and Tyrosine (TK) residues, respectively.
Over 188 kinases have been validated with this technique and the assay validation section of this page includes technical tips for each assay setup.
The Discovery kit is intended to help determine which of the three STK kits is appropriate when working with a non-validated Serine/Threonine kinase.
Benefits
- EASY KINASE PRE-VALIDATION
- SEMI-UNIVERSAL METHOD FOR STKS
- LOW ENZYME CONSUMPTION
Assay principle
Each HTRF KinEASE kit is based on the same assay format, and uses a universal biotinylated substrate, a phospho-specific Eu-cryptate-labeled monoclonal antibody, and SA-XL665. All three STK peptidic substrates are made of the same sequence for antibody detection, next to a variable sequence recognized by enzymes. The targeted residue for phosphorylation lies in between as part of both sequences for enzymatic activity (unphosphorylated) and antibody detection (phosphorylated). It has been shown that 3 different variable sequences could enable the study of most Serine/Threonine kinases.
Assay principle
As shown here, all HTRF KinEASE assays work the same way and involve two steps:
Step 1: Kinase reaction The kinase is incubated in the presence or absence of compounds and the appropriate substrate. The supplemented enzymatic buffer is added in the kinase buffer for tyr kinases only. ATP is added to start the reaction.
Step 2: Detection Eu3+ cryptate and XL665 conjugates are added. The detection buffer contains EDTA to stop the enzymatic reaction.
Validated kinases
Click in the table below to view the list of validated enzymes, the kit which use is recommended, and download the technical datasheet including experimental setting and validation data. Non listed STKs can be assessed with the Discovery kit to find out which HTRF KinEASE STK kit may be used.
Over 190 Serine/Threonine and Tyrosine kinases have already been validated with the HTRF KinEASE kits, and their optimal substrate determined. Note that one given enzyme may be compatible with several substrates, as shown here with MST1 that work equally well with S3 and S2, but significantly less with S1.
Universal platform to address Ser/Thr & Tyr kinases - Application Notes
KinEASE, a universal expanded platform to adress Ser/Thr & Tyr kinases
Measuring kinase activity under any conditions - Application Notes
Exploration is easier with a trusted partner
HTRF KinEASE Tyr and Ser/Thr kits - Flyers
Validated kinases and assay conditions - Flyers
Product Insert KinEASE STK Disc Kit / 62ST0PEB
62ST0PEB - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
The essential white paper to expand your knowledge on kinases
Learn about cutting edge kinase research in this free white paper - Guides
Multiple therapeutic areas adressed with HTRF KinEase assays
Discover how peer researchers challenge kinase investigations with KinEase assays - Application Notes
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Revolutionize your kinases investigations with HTRF
Watch this video to learn more about kinases investigations - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Signaling pathways in liquid tumors
This litterature review highlights the signaling pathways and give examples of how HTRF® KinEASE™ and AlphaLISA® SureFire® assays can be used in the development of novel therapies for leukemia treatment - Application Notes
From biochemical to cell based - Brochures
Safety Data Sheet KinEASE STK Disc Kit / 62ST0PEB
62ST0PEB - Safety Data Sheet
Safety Data Sheet (DEU) KinEASE STK Disc Kit / 62ST0PEB
62ST0PEB - Safety Data Sheet
Safety Data Sheet (ELL) KinEASE STK Disc Kit / 62ST0PEB
62ST0PEB - Safety Data Sheet
Safety Data Sheet (FRA-FR) KinEASE STK Disc Kit / 62ST0PEB
62ST0PEB - Safety Data Sheet
Safety Data Sheet (ITA) KinEASE STK Disc Kit / 62ST0PEB
62ST0PEB - Safety Data Sheet
Safety Data Sheet (SPA) KinEASE STK Disc Kit / 62ST0PEB
62ST0PEB - Safety Data Sheet
Safety Data Sheet (ENG-GB) KinEASE STK Disc Kit / 62ST0PEB
62ST0PEB - Safety Data Sheet
Safety Data Sheet (ENG-US) KinEASE STK Disc Kit / 62ST0PEB
62ST0PEB - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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