Human CCL5 (RANTES) kit HTRF®

The HTRF human CCL5 (RANTES) kit is designed for the quantification of human CCL5 release in cell supernatant.

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  • No-wash No-wash
  • Low sample consumption Low sample consumption
  • Under 2h bench time Under 2h bench time

The HTRF human CCL5 (RANTES) kit is designed for the quantification of human CCL5 release in cell supernatant.



CCL5, also known as RANTES (regulated upon activation, normal T-cell expressed and secreted), is a chemokine mainly secreted by T lymphocytes, macrophages, platelets, and certain types of tumor cells. RANTES plays an important role in inflammatory sites by recruiting T cells and macrophages. When secreted with IL-2 and IFN-γ, RANTES also induces the activation and proliferation of natural killer (NK) cells. The function of RANTES in oncology is still unclear, but in infectious diseases RANTES has been described as inhibiting HIV entry into target cells.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human CCL5/RANTES AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.



Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
CCL5 kit assay principle

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your CCL5 analysis.

Technical specifications of human CCL5 kit

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol
LOD &LOQ (in Diluent) 7 pg/mL &18 pg/mL
Range 18 – 2,000 pg/mL
Time to result ON @ RT
Calibration NIBSC (92/520) value (IU/mL) = 0,015 x HTRF hCCL5 value (pg/mL)
Species Human only

Intra and Inter assay

Intra assay (n=24)

Sample Mean [CCL5] (pg/mL) CV
1 74 7%
2 417 7%
3 1047 6%
Mean CV 7%

Inter assay (n=4)

Sample [CCL5] (pg/mL) Mean (delta ratio) CV
1 62 320 12%
2 256 1214 13%
3 1008 4405 5%
Mean CV 10%

CCL5 secretion in PBMCs stimulated with LPS, IL1ß and TNFa

PBMCs were plated at 100,000 cells/well in a 96 well plate, and stimulated for 24h with LPS, IL1ß and TNFa respectively used at 200 µg/mL, 10 ng/mL and 10 ng/mL. Supernatants were diluted to 1/3, and 16 µL of the diluted samples were transferred into a 384 well (SV) white detection plate to be analyzed by with the Human CCL5 Assay.
CCL5 secretion in PBMCs stimulated with LPS, IL1ß and TNFa.

CCL5 secretion in LX2 stimulated with TGFß

LX2 cells were plated at 400,000 cells/well in a 6 well plate, cultured for 3 days, then starved for 1 day before 48 hours of stimulation with TGFß at 0.5ng/ml. Next, 16 µL of supernatant were transferred into a 384 well (SV) white detection plate to be analyzed with the Human CCL5 Assay.

CCL5 secretion in LX2 cells stimulated with TGFß.

HTRF Cytokine cell-based assays

Monitor immune regulation the simple way - Flyers

HTRF assays for Oncology and Inflammation

Signaling in the Immune System - Brochures

HTRF measurement of cytokine release from fresh blood samples

In collaboration with Blood assay solution - Application Notes

Cytokine secretion profile on PBMCs

One sample to measure them all - Posters

Guidelines from PBMC isolation to cytokine assay optimisation

Perform and optimize cytokine assays on PBMC - Technical Notes

How to freeze PBMC

Guideline for PBMC freezing - Technical Notes

How to measure multiple cytokines from 1 sample

What to expect at the bench - Videos

HTRF makes cytokine detection easy

A fun video introducing you to cytokines assays with HTRF - Videos

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

Cytokine assays: a guide to success with HTRF

Setting up a successful cytokine assay - Guides

Portfolio of Life Sciences Cytokine Assays

This leaflet presents the analytical performances of each assay - Brochures

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Innovative solutions in Virology research

Kits and reagents for virology research - Flyers

Assays for autoimmunity research

Advance your research on autoimmune diseases - Flyers

Manual CCL5 (h) Kit / 62hccl5peg-62hccl5peh

62hccl5peg-62hccl5peh - Product Insert

Certificate of Analysis CCL5 (h) Kit / 62HCCL5PEG

62HCCL5PEG Batch 03D - Quality Control Report

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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