MAb Anti-phospho ELK-1-Eu cryptate HTRF®

MAb Anti-phospho ELK-1-Eu cryptate enables the rapid detection of ELK phosphorylation on Ser383 in a biochemical kinase assay format.
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  • No-wash No-wash
  • Ease-of-use Ease-of-use
  • High affinity High affinity
MAb Anti-phospho ELK-1-Eu cryptate enables the rapid detection of ELK phosphorylation on Ser383 in a biochemical kinase assay format.


The Anti-phospho ELK1-Eu cryptate is a labeled monoclonal antibody enabling the rapid detection of ELK1 only when phosphorylated at Ser383, it does not cross-react with the corresponding non-phosphorylated sequence. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.



Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
HTRF kinase assay principle

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phospho ELK-1-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.
HTRF kinase assay protocol

Open R&D: Sanofi Access Platform

In collaboration with Sanofi - Scientific Presentations

Experience the freedom of building your own assay

HTRF kinase activity toolbox reagents - Flyers

Product Insert A-P ELK-Eu / 61P11KAE-61P11KAZ-61P11KAY

61P11KAE-61P11KAZ-61P11KAY - Product Insert

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

The essential white paper to expand your knowledge on kinases

Learn about cutting edge kinase research in this free white paper - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Revolutionize your kinases investigations with HTRF

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Easy method for kinetic binding determination

How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes

HTRF solutions for Kinases

From biochemical to cell based - Brochures

Safety Data Sheet A-P ELK-Eu / 61P11KAE

61P11KAE - Safety Data Sheet

Batch Information A-P ELK-Eu / 20230726

61P11KAE Batch 06 - Batch Information

Batch Information A-P ELK-Eu / 20240118

61P11KAE Batch 05 - Batch Information

Safety Data Sheet A-P ELK-Eu / 61P11KAY

61P11KAY - Safety Data Sheet

Safety Data Sheet A-P ELK-Eu / 61P11KAZ

61P11KAZ - Safety Data Sheet

Batch Information A-P ELK-Eu / 20230726

61P11KAZ Batch 06 - Batch Information

Plate Reader Requirement

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