A method for determining enzyme kinetic parameters (Km, Kcat) using HTRF...
MAb Anti-phospho I kappa B alpha-Eu cryptate HTRF®
MAb Anti-phospho I kappa B alpha-Eu cryptate enables the rapid detection of I kappa B phosphorylation on Ser32/36 in a biochemical kinase assay format.
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No-wash
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Ease-of-use
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High affinity
Overview
The Anti-phospho I kappa B alpha-Eu cryptate is a labeled monoclonal antibody enabling the rapid detection of I Kappa B alpha only when phosphorylated at Ser32/36, it does not cross-react with the corresponding non-phosphorylated sequence. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
Benefits
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-IKBALPHA (SER32/36) SPECIFIC
Assay principle
HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
Assay protocol
HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phospho I kappa B alpha-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
Experience the freedom of building your own assay
HTRF kinase activity toolbox reagents - Flyers
Product Insert A-P IkBa-Eu / 61P06KAE-61P06KAZ-61P06KAY
61P06KAE-61P06KAZ-61P06KAY - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
The essential white paper to expand your knowledge on kinases
Learn about cutting edge kinase research in this free white paper - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Revolutionize your kinases investigations with HTRF
Watch this video to learn more about kinases investigations - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
From biochemical to cell based - Brochures
Safety Data Sheet A-P IkBa-Eu / 61P06KAE
61P06KAE - Safety Data Sheet
Safety Data Sheet (DEU) A-P IkBa-Eu / 61P06KAE
61P06KAE - Safety Data Sheet
Safety Data Sheet (ELL) A-P IkBa-Eu / 61P06KAE
61P06KAE - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P IkBa-Eu / 61P06KAE
61P06KAE - Safety Data Sheet
Safety Data Sheet (ITA) A-P IkBa-Eu / 61P06KAE
61P06KAE - Safety Data Sheet
Safety Data Sheet (SPA) A-P IkBa-Eu / 61P06KAE
61P06KAE - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P IkBa-Eu / 61P06KAE
61P06KAE - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P IkBa-Eu / 61P06KAE
61P06KAE - Safety Data Sheet
Batch Information A-P IkBa-Eu / 20230804
61P06KAE Batch 03B - Batch Information
Safety Data Sheet A-P IkBa-Eu / 61P06KAY
61P06KAY - Safety Data Sheet
Safety Data Sheet (DEU) A-P IkBa-Eu / 61P06KAY
61P06KAY - Safety Data Sheet
Safety Data Sheet (ELL) A-P IkBa-Eu / 61P06KAY
61P06KAY - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P IkBa-Eu / 61P06KAY
61P06KAY - Safety Data Sheet
Safety Data Sheet (ITA) A-P IkBa-Eu / 61P06KAY
61P06KAY - Safety Data Sheet
Safety Data Sheet (SPA) A-P IkBa-Eu / 61P06KAY
61P06KAY - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P IkBa-Eu / 61P06KAY
61P06KAY - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P IkBa-Eu / 61P06KAY
61P06KAY - Safety Data Sheet
Batch Information A-P IkBa-Eu / 20230804
61P06KAY Batch 03 - Batch Information
Safety Data Sheet A-P IkBa-Eu / 61P06KAZ
61P06KAZ - Safety Data Sheet
Safety Data Sheet (DEU) A-P IkBa-Eu / 61P06KAZ
61P06KAZ - Safety Data Sheet
Safety Data Sheet (ELL) A-P IkBa-Eu / 61P06KAZ
61P06KAZ - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P IkBa-Eu / 61P06KAZ
61P06KAZ - Safety Data Sheet
Safety Data Sheet (ITA) A-P IkBa-Eu / 61P06KAZ
61P06KAZ - Safety Data Sheet
Safety Data Sheet (SPA) A-P IkBa-Eu / 61P06KAZ
61P06KAZ - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P IkBa-Eu / 61P06KAZ
61P06KAZ - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P IkBa-Eu / 61P06KAZ
61P06KAZ - Safety Data Sheet
Batch Information A-P IkBa-Eu / 20230804
61P06KAZ Batch 03 - Batch Information
Plate Reader Requirement
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