A method for determining enzyme kinetic parameters (Km, Kcat) using HTRF...
MAb Anti-phospho P38-Eu cryptate HTRF®
MAb Anti-phospho P38-Eu cryptate enables the rapid detection of p38 MAPK phosphorylation on Thr180 & Tyr182 in a biochemical kinase assay format.
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No-wash
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Ease-of-use
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High affinity
Overview
The Anti-phospho p38-Eu cryptate is a labeled MAb enabling the detection of p38 MAPK only when phosphorylated at Thr180 & Tyr 182. It does not cross-react with the corresponding phosphorylated forms of either p44/42 MAPK or SAPK/JNK . It does not detect non-phosphorylated p38 MAPK. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
Benefits
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-P38 (THR180/TYR182) SPECIFIC
Assay principle
HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
Assay protocol
HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phospho P38-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
Experience the freedom of building your own assay
HTRF kinase activity toolbox reagents - Flyers
Product Insert A-P P38-Eu / 61P04KAE-61P04KAZ-61P04KAY
61P04KAE-61P04KAZ-61P04KAY - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
The essential white paper to expand your knowledge on kinases
Learn about cutting edge kinase research in this free white paper - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Revolutionize your kinases investigations with HTRF
Watch this video to learn more about kinases investigations - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
From biochemical to cell based - Brochures
Safety Data Sheet A-P P38-Eu / 61P04KAE
61P04KAE - Safety Data Sheet
Safety Data Sheet (DEU) A-P P38-Eu / 61P04KAE
61P04KAE - Safety Data Sheet
Safety Data Sheet (ELL) A-P P38-Eu / 61P04KAE
61P04KAE - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P P38-Eu / 61P04KAE
61P04KAE - Safety Data Sheet
Safety Data Sheet (ITA) A-P P38-Eu / 61P04KAE
61P04KAE - Safety Data Sheet
Safety Data Sheet (SPA) A-P P38-Eu / 61P04KAE
61P04KAE - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P P38-Eu / 61P04KAE
61P04KAE - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P P38-Eu / 61P04KAE
61P04KAE - Safety Data Sheet
Batch Information A-P P38-Eu / 20240406
61P04KAE Batch 09 - Batch Information
Batch Information A-P P38-Eu / 20240802
61P04KAE Batch 06 - Batch Information
Safety Data Sheet A-P P38-Eu / 61P04KAY
61P04KAY - Safety Data Sheet
Safety Data Sheet (DEU) A-P P38-Eu / 61P04KAY
61P04KAY - Safety Data Sheet
Safety Data Sheet (ELL) A-P P38-Eu / 61P04KAY
61P04KAY - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P P38-Eu / 61P04KAY
61P04KAY - Safety Data Sheet
Safety Data Sheet (ITA) A-P P38-Eu / 61P04KAY
61P04KAY - Safety Data Sheet
Safety Data Sheet (SPA) A-P P38-Eu / 61P04KAY
61P04KAY - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P P38-Eu / 61P04KAY
61P04KAY - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P P38-Eu / 61P04KAY
61P04KAY - Safety Data Sheet
Batch Information A-P P38-Eu / 20240406
61P04KAY Batch 09 - Batch Information
Safety Data Sheet A-P P38-Eu / 61P04KAZ
61P04KAZ - Safety Data Sheet
Safety Data Sheet (DEU) A-P P38-Eu / 61P04KAZ
61P04KAZ - Safety Data Sheet
Safety Data Sheet (ELL) A-P P38-Eu / 61P04KAZ
61P04KAZ - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P P38-Eu / 61P04KAZ
61P04KAZ - Safety Data Sheet
Safety Data Sheet (ITA) A-P P38-Eu / 61P04KAZ
61P04KAZ - Safety Data Sheet
Safety Data Sheet (SPA) A-P P38-Eu / 61P04KAZ
61P04KAZ - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P P38-Eu / 61P04KAZ
61P04KAZ - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P P38-Eu / 61P04KAZ
61P04KAZ - Safety Data Sheet
Batch Information A-P P38-Eu / 20240406
61P04KAZ Batch 09 - Batch Information
Plate Reader Requirement
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