HTRF KinEASE STK Discovery kit
A assay to determine which HTRF KinEASE STK kit must be used
MAb PY20-Eu cryptate HTRF®
MAb PY20-Eu cryptate enables the rapid detection of phosphorylation of peptides or proteins on Tyrosine in a biochemical kinase assay format.
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No-wash
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Ease-of-use
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High affinity
Overview
PY20-Eu cryptate is a labeled mouse monoclonal IgG2b. It shows to react specifically with phosphorylated tyrosine, both as free amino acid or when conjugated to carriers. No cross-reactivity has been observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP. This allows the construction of a highly specific biochemical Tyrosine kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.
Benefits
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-(TYR) SPECIFIC
Assay principle
HTRF Tyrosine kinase assay use typically a combination of a Europium-cryptate labeled anti-phosphotyrosine antibody, SA-XL665 and a biotinylated substrate. The signal is proportional to the concentration of phospho-residues. Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
Assay protocol
HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb PY20-Eu cryptate prepared in a buffer containing EDTA to stop the enzymatic reaction.
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
Experience the freedom of building your own assay
HTRF kinase activity toolbox reagents - Flyers
Product Insert PY20-Eu / 61Y20KLA-61Y20KLB
61Y20KLA-61Y20KLB - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
The essential white paper to expand your knowledge on kinases
Learn about cutting edge kinase research in this free white paper - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Revolutionize your kinases investigations with HTRF
Watch this video to learn more about kinases investigations - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
From biochemical to cell based - Brochures
Batch Information PY20-Eu / 20251224
61Y20KLA Batch 26J - Batch Information
Safety Data Sheet PY20-Eu / 61Y20KLA
61Y20KLA - Safety Data Sheet
Safety Data Sheet (DEU) PY20-Eu / 61Y20KLA
61Y20KLA - Safety Data Sheet
Safety Data Sheet (ELL) PY20-Eu / 61Y20KLA
61Y20KLA - Safety Data Sheet
Safety Data Sheet (FRA-FR) PY20-Eu / 61Y20KLA
61Y20KLA - Safety Data Sheet
Safety Data Sheet (ITA) PY20-Eu / 61Y20KLA
61Y20KLA - Safety Data Sheet
Safety Data Sheet (SPA) PY20-Eu / 61Y20KLA
61Y20KLA - Safety Data Sheet
Safety Data Sheet (ENG-GB) PY20-Eu / 61Y20KLA
61Y20KLA - Safety Data Sheet
Safety Data Sheet (ENG-US) PY20-Eu / 61Y20KLA
61Y20KLA - Safety Data Sheet
Batch Information PY20-Eu / 20231029
61Y20KLA Batch 32A - Batch Information
Batch Information PY20-Eu / 20231120
61Y20KLA Batch 33A - Batch Information
Batch Information PY20-Eu / 20240215
61Y20KLA Batch 35A - Batch Information
Safety Data Sheet PY20-Eu / 61Y20KLB
61Y20KLB - Safety Data Sheet
Safety Data Sheet (FRA-FR) PY20-Eu / 61Y20KLB
61Y20KLB - Safety Data Sheet
Safety Data Sheet (ITA) PY20-Eu / 61Y20KLB
61Y20KLB - Safety Data Sheet
Safety Data Sheet (SPA) PY20-Eu / 61Y20KLB
61Y20KLB - Safety Data Sheet
Safety Data Sheet (ENG-GB) PY20-Eu / 61Y20KLB
61Y20KLB - Safety Data Sheet
Safety Data Sheet (ENG-US) PY20-Eu / 61Y20KLB
61Y20KLB - Safety Data Sheet
Safety Data Sheet (DEU) PY20-Eu / 61Y20KLB
61Y20KLB - Safety Data Sheet
Safety Data Sheet (ELL) PY20-Eu / 61Y20KLB
61Y20KLB - Safety Data Sheet
Batch Information PY20-Eu / 20231120
61Y20KLB Batch 33A - Batch Information
Batch Information PY20-Eu / 20231211
61Y20KLB Batch 34A - Batch Information
Batch Information PY20-Eu / 20240215
61Y20KLB Batch 35A - Batch Information
Batch Information PY20-Eu / 20240714
61Y20KLB Batch 36A - Batch Information
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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