HTRF KinEASE-STK S3 kit
A HTRF KinEASE kit to study serine/threonine kinases
PAb Anti-phospho ATF2-Eu cryptate HTRF®
PAb Anti-phospho ATF2-Eu cryptate enables the rapid detection of ATF2 phosphorylation on Thr69/71 in a biochemical kinase assay format.
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No-wash
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Ease-of-use
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High affinity
Overview
The Anti-phospho ATF2-Eu cryptate is a labeled polyclonal antibody enabling the detection of ATF2 when singly phosphorylated at Ser 71 or dually phosphorylated at Thr69/71. It does not detect the non-phosphorylated ATF2. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
Benefits
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-ATF2 (THR71) SPECIFIC
Assay principle
HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
Assay protocol
HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Anti-phospho ATF2-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
Experience the freedom of building your own assay
HTRF kinase activity toolbox reagents - Flyers
Product Insert A-P ATF2-Eu / 61P12KAE-61P12KAZ-61P12KAY
61P12KAE-61P12KAZ-61P12KAY - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
The essential white paper to expand your knowledge on kinases
Learn about cutting edge kinase research in this free white paper - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Revolutionize your kinases investigations with HTRF
Watch this video to learn more about kinases investigations - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
From biochemical to cell based - Brochures
Safety Data Sheet A-P ATF2-Eu / 61P12KAE
61P12KAE - Safety Data Sheet
Safety Data Sheet (DEU) A-P ATF2-Eu / 61P12KAE
61P12KAE - Safety Data Sheet
Safety Data Sheet (ELL) A-P ATF2-Eu / 61P12KAE
61P12KAE - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P ATF2-Eu / 61P12KAE
61P12KAE - Safety Data Sheet
Safety Data Sheet (ITA) A-P ATF2-Eu / 61P12KAE
61P12KAE - Safety Data Sheet
Safety Data Sheet (SPA) A-P ATF2-Eu / 61P12KAE
61P12KAE - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P ATF2-Eu / 61P12KAE
61P12KAE - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P ATF2-Eu / 61P12KAE
61P12KAE - Safety Data Sheet
Batch Information A-P ATF2-Eu / 20230914
61P12KAE Batch 05G - Batch Information
Batch Information A-P ATF2-Eu / 20320703
61P12KAE Batch 05H - Batch Information
Batch Information A-P ATF2-Eu / 20230914
61P12KAE Batch 05H - Batch Information
Safety Data Sheet A-P ATF2-Eu / 61P12KAY
61P12KAY - Safety Data Sheet
Safety Data Sheet (DEU) A-P ATF2-Eu / 61P12KAY
61P12KAY - Safety Data Sheet
Safety Data Sheet (ELL) A-P ATF2-Eu / 61P12KAY
61P12KAY - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P ATF2-Eu / 61P12KAY
61P12KAY - Safety Data Sheet
Safety Data Sheet (ITA) A-P ATF2-Eu / 61P12KAY
61P12KAY - Safety Data Sheet
Safety Data Sheet (SPA) A-P ATF2-Eu / 61P12KAY
61P12KAY - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P ATF2-Eu / 61P12KAY
61P12KAY - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P ATF2-Eu / 61P12KAY
61P12KAY - Safety Data Sheet
Batch Information A-P ATF2-Eu / 20230914
61P12KAY Batch 05 - Batch Information
Safety Data Sheet A-P ATF2-Eu / 61P12KAZ
61P12KAZ - Safety Data Sheet
Safety Data Sheet (DEU) A-P ATF2-Eu / 61P12KAZ
61P12KAZ - Safety Data Sheet
Safety Data Sheet (ELL) A-P ATF2-Eu / 61P12KAZ
61P12KAZ - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P ATF2-Eu / 61P12KAZ
61P12KAZ - Safety Data Sheet
Safety Data Sheet (ITA) A-P ATF2-Eu / 61P12KAZ
61P12KAZ - Safety Data Sheet
Safety Data Sheet (SPA) A-P ATF2-Eu / 61P12KAZ
61P12KAZ - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P ATF2-Eu / 61P12KAZ
61P12KAZ - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P ATF2-Eu / 61P12KAZ
61P12KAZ - Safety Data Sheet
Batch Information A-P ATF2-Eu / 20230914
61P12KAZ Batch 05H - Batch Information
Batch Information A-P ATF2-Eu / 20320703
61P12KAZ Batch 05I - Batch Information
Batch Information A-P ATF2-Eu / 20230914
61P12KAZ Batch 05I - Batch Information
Plate Reader Requirement
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