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Procollagen-III-peptide (PIIINP) ELISA kit

Procollagen-III-peptide (PIIINP) ELISA kit ELISA

The P3NP-EL-US test enables the determination of human procollagen III peptide (P3NP) in blood for fibrosis research.
For Research Use Only, not for use in diagnostic procedures.

Benefits

One-step ELISA assay at RT
Automation friendly

Assay principle: sandwich

The P3NP-EL-US assay is a colorimetric immunoassay. A monoclonal antibody, immobilized on the microplate, captures the PIIINP proteins contained in the calibrators and samples. The bound proteins are then recognized by a second monoclonal antibody conjugated to HRP (Horse-Radish peroxidase). The unbound reagents are eliminated by washing. Then, the colorimetric reaction is started by the addition of the HRP substrate TMB. The reaction is stopped by addition of an acid solution, the optical density (OD) of each well is read at 450 nm.