Zinc transporter 8 autoantibody (ZnT8Ab) ELISA kit ELISA

  • Photography of ZNT8 AB ELISA
  • Photography of ZNT8 AB ELISA
The ZNT8/96 kit enables a quantitative determination in human serum of zinc transporter 8 autoantibodies associated with type I diabetes mellitus.
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The ZNT8/96 kit enables a quantitative determination in human serum of zinc transporter 8 autoantibodies associated with type I diabetes mellitus.
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Overview

Quantify ZnT8 autoantibodies specific to R 325 or to W 325 or to residue 325 non specific variants

Use as serological markers in type 1 diabetes mellitus

Benefits

  • ACCURATE AND RELIABLE ENZYMATIC ASSAY

Assay principle: sandwich

ZnT8 molecules are coated onto the ELISA plate wells. ZnT8-biotin complexes constitute the liquid phase. ZnT8-Ab from the sample bound to the ZnT8 coated onto the well will also interact with ZnT8-biotin to form a bridge. Addition of streptavidin peroxidase (SA-POD) detects the bound ZnT8-biotin. The unbound SA-POD in excess is washed off before the addition of the peroxidase substrate TMB.

Assay procedure: Overnight (16h-20h) at 2-8°C + 1 h at 2-8°C + 20 min RT / shaking + 20 min TA in the dark.

Features

Working range: 10 – 2000 U/mL

Sample: serum – 25 µL

Analytical sensitivity: 1.2 U/mL

Normal values

Cut-offZnT8Ab U/mL
Negative<15

Certificate of Analysis ZnT8 / ZNT8--96

ZNT8/96 Batch KZTE135 - Quality Control Report